WB实验是我们科研生活中常做的一项实验，严谨细致的实验操作，有助于我们获得可信的实验结果。在进行写文章投稿时，我们需要对原始结果进行整理排版。接下来教大家如何用photoshop软件制作WB实验结果图，在不影响实验结果的前提下，可以修改对比度，使图片更美观;同时还可以通过修剪使条带边缘排列整齐。下面的图片就是小编用Photoshop做的WB图。
一般处理图片还是用专业的图片软件处理比较好，毕竟很少有杂志接收PPT形式的图片的，一般都要求TIFF或者PDF格式的。我们在处理图片的时候一定要遵守杂志对其的要求。比如可以处理图片的什么内容、要求多少d.p.i.等。我们可以看一下Nature杂志对于图片处理的T投稿要求：
Image integrity and standards
Images submitted with a manuscript for review should be minimally processed (for instance, to add arrows to a micrograph). Authors should retain their unprocessed data and metadata files, as editors may request them to aid in manuscript evaluation. If unprocessed data are unavailable, manuscript evaluation may be stalled until the issue is resolved. All digitized images submitted with the final revision of the manuscript must be of high quality and have resolutions of at least 300 d.p.i. for colour, 600 d.p.i. for greyscale and 1,200 d.p.i. for line art.
A certain degree of image processing is acceptable for publication (and for some experiments, fields and techniques is unavoidable), but the final image must correctly represent the original data and conform to community standards. The guidelines below will aid in accurate data presentation at the image processing level; authors must also take care to exercise prudence during data acquisition, where misrepresentation must equally be avoided.
Authors should list all image acquisition tools and image processing software packages used. Authors should document key image-gathering settings and processing manipulations in the Methods.
Images gathered at different times or from different locations should not be combined into a single image, unless it is stated that the resultant image is a product of time-averaged data or a time-lapse sequence. If juxtaposing images is essential, the borders should be clearly demarcated in the figure and described in the legend.
The use of touch-up tools, such as cloning and healing tools in Photoshop, or any feature that deliberately obscures manipulations, is to be avoided.
Processing (such as changing brightness and contrast) is appropriate only when it is applied equally across the entire image and is applied equally to controls. Contrast should not be adjusted so that data disappear. Excessive manipulations, such as processing to emphasize one region in the image at the expense of others (for example, through the use of a biased choice of threshold settings), is inappropriate, as is emphasizing experimental data relative to the control.
从Nature的要求中我们可以看到，一般彩色图片要求300 d.p.i.，灰度图片要求600 d.p.i.，而line art(比如绘制的柱形图、饼状图等)则要求1200 d.p.i.，由此，我们可以看出基本300 d.p.i.就可以满足大部分要求了。另外一般杂志都是可以接受Photoshop等对图片进行亮度、对比度、色彩平衡等进行调节的，当然是要整体进行调整，不能改变结果的原有趋势。下面小编就讲解一下如何用Photoshop对WB图片进行处理。
首先在Photoshop中打开所要处理的WB图片;
2. 新建一个窗口，关于尺寸的选择一般杂志也都有要求，比如Cell杂志就对图片尺寸做了详细的要求。
https://www.cell.com/figureguidelines?code=cell-site
这个尺寸就是要求一个figure不能超过8.5×11英寸，这个尺寸是美国信纸letter的标准尺寸，在Photoshop里有现成的模板，我们直接选择这个尺寸：
3. 然后选中我们打开的WB图片窗口，选择“图像”-“模式”-“灰度”，将我们的图片变为灰度图片;
4. 选择左侧“矩形选框工具”;
5. 在WB图片上选中我们需要的区域，然后在左侧再点击一下“移动工具”，之后将选择的区域拖曳到新建的空白模板上
6. 然后给图片加个边框：选择“编辑”-“描边”即可。科研小助手一般选择4-6个像素，加在外侧。
7. 然后再添加必要的文字说明即可完成了：
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